• Alterations in genetically modified crops assessed by omics studies: Systematic review and meta-analysis 

      Benevenuto, Rafael Fonseca; Venter, Hermoine Jean; Zanatta, Caroline Bedin; Nodari, Rubens Onofre; Agapito-Tenfen, Sarah Zanon (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-01-04)
      Background: International agreements and domestic legislation regulate genetically modified (GM) crops for environmental release, recognizing that genetic engineering could result in unintended genotypic and phenotypic effects. In that context, omics technologies, which allow comprehensive characterization of the molecular profile of GM crops at all levels, may be used to assess alterations or ...
    • Alterations in genetically modified crops assessed by omics studies: Systematic review and meta-analysis 

      Benevenuto, Rafael Fonseca; Venter, Hermoine Jean; Zanatta, Caroline Bedin; Nodari, Rubens Onofre; Agapito-Tenfen, Sarah Zanon (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-01-04)
      Background International agreements and domestic legislation regulate genetically modified (GM) crops for environmental release, recognizing that genetic engineering could result in unintended genotypic and phenotypic effects. In that context, omics technologies, which allow comprehensive characterization of the molecular profile of GM crops at all levels, may be used to assess alterations or effects ...
    • A DNA-Free Editing Platform for Genetic Screens in Soybean via CRISPR/Cas9 Ribonucleoprotein Delivery 

      Subburaj, Saminathan; Zanatta, Caroline Bedin; Nunn, Jennifer Ann Lillebo; Hoepers, Aline Martins; Nodari, Rubens Onofre; Agapito-Tenfen, Sarah Zanon (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-07-12)
      CRISPR/Cas9-based ribonucleoprotein (RNP)-mediated system has the property of minimizing the effects related to the unwanted introduction of vector DNA and random integration of recombinant DNA. Here, we describe a platform based on the direct delivery of Cas9 RNPs to soybean protoplasts for genetic screens in knockout gene-edited soybean lines without the transfection of DNA vectors. The platform ...